The objective of this study was to determine whether the performance of mice in a battery of three behavioral tests was affected by the sequence of the tests. The test battery included the open field test (OFT), elevated plus maze (EPM), and tail suspension test (TST). Young adult, female mice were randomly assigned to one of six treatment groups. The sequence of the tests varied for each group. The behavioral parameters measured in each test included those reported in the scientific literature, such as the time spent in the center of the OFT box, the time spent on the open arms of the EPM, and immobility time in the TST. Statistical analyses (ANOVA and Tukey’s t-tests) were performed to detect any treatment effects. However, the results revealed no significant differences among the treatment groups for the behavioral parameters examined. Therefore, we conclude that the sequence of the tests in the test battery was not an important factor that affected the animals’ behavior.
